Rewrite $ 16x^2-9y^2 $ as:
$$ \color{blue}{ 16x^2-9y^2 = (4x)^2 - (3y)^2 } $$Now we can apply the difference of squares formula.
$$ I^2 - II^2 = (I - II)(I + II) $$After putting $ I = 4x $ and $ II = 3y $ , we have:
$$ 16x^2-9y^2 = (4x)^2 - (3y)^2 = ( 4x-3y ) ( 4x+3y ) $$